Effect Of Temperature On Hydrolysis Of Cellulose (Saw-Dust)
The effect of concentration of hydrochloric acid on hydrolysis of cellulose (saw-
dust) to glucose was studied on this research project and the steps obtained to
achieve this project involved treatment of saw-dust (cellulose) with different
concentrations of the acid at constant temperature of 80°𝐶 (350k) for 30mins. This
was followed by glucose analysis, some analysis or experiments were done on acid
hydrolysis in order to study the effect of (HCL) acid on the hydrolysis of cellulose
to glucose. The process used in this hydrolysis was acid hydrolysis in which HCL
acid was used at constant temperature of 80oC and the saw-dust used [was obtained
by grinding wood with saw] was weighed and mixed with water . Secondly, during
this analysis/experiment, it was observed that hydrochloric acid hydrolyzed well
from the readings gotten from each result that was carried out during the analysis.
Then lastly, glucose analysis was carried out to determine the absorbance and
glucose concentration. It was noticed that the best concentration of HCL acid
during hydrolysis yields glucose concentration of 0.127g or 1.270%.
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CHAPTER ONE
1.1 Introduction 1
1.2 Definition of Terms 3
1.3 Statement of the Problem 4
1.4 Scope and of Study Limitations 5
1.5 Objectives 6
CHAPTER TWO
2.1 Literature Review 8
2.2 History 9
2.3 Products 10
2.4 Cellulose Source and Energy Store of Crops 12
2.5 Structure and Properties 14
2.6 Biosynthesis 18
2.7 Breakdown (Cellucolysis) 22
2.8 Hemicellulose 24
2.9 Derivatives 24
2.10 Functionality 29
2.11 Occurrences 30
2.12 Classification of Cellulose 31
2.12.1 Cellulose Acetate. 32
2.12.2 Cellulose Acetate Butyrate. 32
2.12.3 Cellulose Nitrate. 33
2 12 .4 Methyl Cellulose.- 34
2.12.5 Ethyl Cellulose. 34
2.12.6 Carboxy Methyl Cellulose. 35
2.13 Regenerated Cellulose. 36
2.14 Uses of Sugar. 38
2.15 Sugar in Foods 40
2.16 Functions of Sugar. 40
2.17 Natural Polymers of Sugar. 41
2.18 Types of Sugar. 42
CHAPTER THREE
3.1 Materials and Equipment 46
3.1.1 Materials: 46
3.1.2 Apparatus: 46
3.1.3 Reagent 47
3.2 Acid Hydrolysis (Hcl) 48
3.2.1 Procedure: 48
3.2.2 Glucose Analysis Colorimetric (Using Benedict’s) Method. 49
3.2.3 Procedure: 50
The Glucose Concentration (Hcl) Was Determined Using
Beer Lambat Law. 50
CHAPTER FOUR
Results and Discussion
4.1 Results 52
4.2 Discussion 53
CHAPTER FIVE
5.0 Conclusion and Recommendation 55
5.1 Conclusion 55
5.2 Recommendation 56
References 58
Appendix A 60
Appendix B 61
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