Biodegradation Of Macro-Polyethylene Plastic Into Micro Particles
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- To characterize the microbial communities associated with polyethylene degradation
- To evaluate the biodegradation efficiency of selected microbial strains
- To investigate the factors influencing microbial plastic degradation
- To assess the biodegradation products and byproducts by analyzing the chemical composition of biodegradation products and byproducts, to identify intermediate metabolites and assess their environmental implications.
Abstract
Chapter One
Introduction
1.1 Plastic Pollution: an Emerging Global Crisis
1.2 Understanding Macro-polyethylene Plastic and Its Environmental Impact
1.3 The Challenge of Biodegradation: Exploring Potential Solutions
1.4 Importance of Microbial Action in Plastic Degradation
1.5 Research Objectives and Scope of the Study
1.6 Definition of Terms
Chapter Two
Literature Review
2.1 Plastic Pollution: Magnitude and Environmental Impacts
2.2 Types of Plastic Polymers and Their Persistence in the Environment
2.3 Mechanisms of Plastic Degradation: Physical, Chemical, and Biological Processes
2.4 Microbial Communities Involved in Plastic Degradation
2.5 Enzymatic Pathways and Biodegradation of Polyethylene Plastics
2.6 Factors Influencing Microbial Plastic Degradation
2.7 Current Challenges and Future Directions in Plastic Pollution Mitigation
Chapter Three
Material and Methods
3.1 Sample Collection and Preparation
3.2 Experimental Design
3.3 Characterization of Plastic Samples
3.4 Microbial Isolation and Culture
3.5 Enzyme Assays
3.6 Biodegradation Experiments
3.7 Analytical Techniques
Chapter Four
Result and Discussions
4.1 Analysis of Biodegradation Rates of Polyethylene Plastics
4.2 Analysis of Enzyme Activities in Microbial Cultures
4.3 Analysis of Effect of Incubation Temperate on Plastic Degradation
4.4 Comparison of Biodegradation Rates in Aerobic and Anaerobic Conditions
4.5 Surface Morphology of Plastic Samples Before and After Degradation
4.6 Identification of Degradation Products by GC-MS Analysis
4.7 Isotopic Labeling Study Results
4.8 Kinetic Parameters of Enzymatic Plastic Degradation
4.9 Discussions of Findings
Chapter Five
Summary, Conclusion and Recommendation
5.1 Summary
5.2 Conclusion
5.3 Recommendation
References
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