Activities Of Cytochrome Oxidase In The Rat Liver Of Normal And Protein Undernourished Rats Fed With Diet Containing Cassava Peel
This research work on “Activities Of Cytochrome Oxidase In The Rat Liver Of Normal And Protein Undernourished Rats Fed With Diet Containing Cassava Peel” is available in PDF/DOC. Click the below button to request or download the complete material
The study was carried out to investigate the activities respiratory complex IV in the liver of normal and protein undernourished wistar rats fed with cassava peel. Twenty weaning male rats were grouped into four groups, five rats for each. Group A were fed with normal diets, Group B were fed with low protein diets containing 5% casein, Group C were fed with normal diets and coconut oil and Group D were fed with low protein diet and coconut oil. After feeding for a period of six weeks, the rats were sacrificed using cervical dislocation. The liver was harvested and homogenized in iced cold buffer and centrifuged at 4000rpm for 15 minutes. The pellets were discarded and the supernatant was stored.
It was observed from this study that consumption of low protein diets significantly reduced the activities of cytochrome oxidase in the rat liver when compared to normal diet. However, the activities of cytochrome oxidase were significantly reduced in the rat liver when the rats were fed with normal and cassava peel diets compared to normal diet only. Also, low protein and cassava peel diets significantly reduced the activities of cytochrome oxidase in the rat liver when compared to normal diet.
It was observed from this study that in the rat liver, the activities of cytochrome oxidase was significantly increased when the rats were fed with normal and cassava peel diets compared to low protein diet only. However, when the rats were fed with low protein diet and cassava peel, the diets significantly increased the activities of cytochrome oxidase compared to both normal and cassava peel and low protein diet only. This study shows that consumption of cassava peel significantly reduced the level of normal diets while it significantly increased the level of low protein diets.
Abstract v
Table of content
Chapter One
1.0 Introduction
1.1 Aim of Study
Chapter Two
2.0 Literature review
2.1 Cassava peel
2.2 Cyanide
2.3 Respiratory Electron Transport Chain
2.3.1 The sequence of electron transport chain
2.3.2 Mitochondria
2.4 Cytochrome oxidase
2.5 Protein undernutrition
2.6 Liver
2.6.1 Synthesis
2.6.2 Liver disease and blood supply
Chapter Three
3.0 Materials and Methods
3.1 Materials
3.1.1 Chemicals and reagents
3.2 Methods
3.2.1 Animal managements
3.2.2 Feed composition
3.2.3 Body weight determination
3.2.4 Animal sacrifice
3.2.5 Isolation of mitochondria
3.3 Determination of complex IV (Cytochrome oxidase activity
3.4 Protein determination
Chapter Four
4.0 Results
Chapter Five
5.0 Discussion and Conclusion
5.1 Discussion
5.2 Conclusion
References
Appendix.
A 150–300 word synopsis of the main objectives, methods, findings, and conclusions of the Activities Of Cytochrome Oxidase In The Rat Liver Of Normal And Protein Undernourished Rats Fed With Diet Containing Cassava Peel should be included in the abstract.
Every chapter, section, and subsection in the research work should be listed in the Table of Contents, including the page numbers that correspond to each one.
The background, research question or hypothesis, and objective or aim of the Activities Of Cytochrome Oxidase In The Rat Liver Of Normal And Protein Undernourished Rats Fed With Diet Containing Cassava Peel should all be presented in the introduction, which is the first section.
A survey of previously conducted research on Activities Of Cytochrome Oxidase In The Rat Liver Of Normal And Protein Undernourished Rats Fed With Diet Containing Cassava Peel should be included in the literature review, together with an overview of the main conclusions, a list of any gaps, and an introduction to the current study.
The conclusion part should address the implications of the study, provide an answer to the research question and summarize the key findings.
The reference of Activities Of Cytochrome Oxidase In The Rat Liver Of Normal And Protein Undernourished Rats Fed With Diet Containing Cassava Peel, which should be formatted following a particular citation style (such as APA, MLA, or Chicago), is a list of all the sources cited in the title.
Other important sections of the Activities Of Cytochrome Oxidase In The Rat Liver Of Normal And Protein Undernourished Rats Fed With Diet Containing Cassava Peel should include the Title page, Dedication, Acknowledgments, Methodology, Results, Discussion, Appendices, Glossary, or Abbreviations List where applicable.