Susceptibilities Of Salmonella Typhi And Other Bacterial Pathogens To Antibiotics And Hot Aqueous Extract Of Hibiscus Sabdariffa
The susceptibilities of Salmonella typhi and other pathogens to antibiotics and hot aqueous extract of Hibiscus sabdariffa were investigated using agar diffusion and agar well diffusion methods respectively. Salmonella typhi was sensitive to ampicillin, cetriaxone, ciprofloxacin, gentamycin of ofloxacin and perfloxacin. Nitrofurantoin, ampicillin, clarithomycin and augumentin are resistant. Escherichia coli, Klebsiella spp, and Staphylococcus aureus were sensitive to 50%, 70% and 60% of the antibiotics respectively. Pseudomonas aeruginosa was resistant to all antibiotics. Hibiscus sabdariffa extract (0.6g in 6ml of sterile distilled water) was active against S. typhi at concentrations of 100mg/ml, 50mg/ml and 25mg/ml (inhibitions zone diameter IZDs = 23mm, 20mm and 16mm respectively). Staphylococcus aureus was susceptible to 100mg/ml, 50mg/ml, 25mg/ml and 12.5mg/ml of the extract with IZDs of 29mm, 18mm, 17mm and 14mm respectively. Klebsiella spp was susceptible to concentrations of 25mg/ml and 12.5mg/ml of the extract with IZDs of 15mm and 10mm respectively. Escherichia coli and Pseudomonas aeruginosa were resistant to all the concentrations of H. sabdariffa extract. It is therefore imperative to note that the use of medicinal plants is recommended to the Government and Industry.
NOTE: Preview the following Table of Content before you download the full content. WYSIWYG – What You See Is What You Get.
i Certification
ii Dedication
iii Acknowledgement
iv Table of content
vi List of tables
ix List of figures
x Appendix
xi Abstract
CHAPTER ONE
1.0 Introduction
1 1. Aims and Objectives
CHAPTER TWO
2.0 Literature review
2.1 Plants extract review
2.1.1 Brief history of medicinal plant
2.1.2 Phytochemical components with antimicrobial activity
2.1.3 Characteristics of phytomedicine
2.1.4 Why the Demand for Phytomedicine
2.1.5 Challenges and Development in the use of phytomedicine
2.1.6 Possible Solutions
2.2 A review of Hibiscus sabdariffa
2.2.1 Scientific classification
2.2.2 Description
2.2.3 Origin and distribution
2.2.4 Various names of H. sabdariffa
2.2.5 Climate
2.2.6 Cultivation and harvesting
2.2.7 Pests and diseases
2.2.8 General uses OF H. sabdariffa
2.2.9 Phytomedicine
2.2.3.1 Various research on H. Sabdariffa
2.3 Description of Salmonella typhi
2.3.1 Scientific classification OF Salmonella typhi
2.3.2 History of typhoid fever
2.3.3 Causative Agent
2.3.4 Mode of transmission
2.3.5 Signs and symptoms
2.3.6 Epidemiology of typhoid fever
2.3.7 Pathogenesis of Typhoid Fever
2.3.8 Prevention and control
2.3.9 Diagnosis
2.34.1 Risk factors
2.3.4.2 Treatment
2.3.4.3 Other pathogens
CHAPTER THREE
3.0 Materials
3.1.1 Hard Equipments
3.1.2 Soft wares
3.1.3 Media used
3.2. Collection and confirmatory of test organisms
3.2.1 Gram staining
3.2.2 Biochemical Tests
3.3 Collection and identification of plant material
3.4 Extraction of plant material
3.5 Preparation of the Mcfarland’s Standard
3.6 Preparation of the cell suspension (inocular)
3.7 Serial dilutions of the extract
3.8 Antibiotics Susceptibility testing
3.9 Susceptibility testing using H. sabdariffa
CHAPTER FOUR
4.1 Results
CHAPTER FIVE
5.1 Discussions of results
5.2 Conclusion
5.3 Recommendation
References
This “Susceptibilities Of Salmonella Typhi And Other Bacterial Pathogens To Antibiotics And Hot Aqueous Extract Of Hibiscus Sabdariffa” file contains 5 Chapters, 113 Pages and 14,769 Words.
The author of Susceptibilities Of Salmonella Typhi And Other Bacterial Pathogens To Antibiotics And Hot Aqueous Extract Of Hibiscus Sabdariffa is written at the front page of the downloaded file.
To download this Susceptibilities Of Salmonella Typhi And Other Bacterial Pathogens To Antibiotics And Hot Aqueous Extract Of Hibiscus Sabdariffa full file, click on “Download File” on top of this page and follow the next.