Kinetic Study On Hydrolysis Of Cellulose (Saw-Dust)
This research project studied on the kinetics of hydrolysis of cellulose to glucose.
The steps employed to achieve this project involved extraction of cellulose from
sawdust and subsequently, hydrolysis of starch to simple sugar. This was followed
by glucose analysis. Different experiments were conducted during acid hydrolysis
to study the various acids on the hydrolysis of cellulose to glucose. The saw-dust
was extracted from the wood by grinding using saw. The process used in the
hydrolysis was acid hydrolysis in which two major inorganic acids (Hydrochloric
and Sulfuric) were used at constant temperature of 80oC. During this experiment, it
was observed that Hydrochloric acid hydrolyzed most, followed by Sulphuric acid.
Finally, sugar analysis was carried out to determine the acid with the highest yield
of glucose and the best acid for the hydrolysis. It was noticed that the yield of
glucose was relatively high from HCl at 1.280% concentration, followed by H2SO4
at 0.940%. It was also seen from the graph that the absorbance yield increases as
the glucose concentrations increases in terms of HCl. Therefore, the best acid for
acid for acid hydrolysis is HCl.
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Title page
Caritas logo
Dedication
Certification
Acknowledgment
Approval page
Abstract
Table of contents
CHAPTER ONE
1.1 Introduction 1
1.2 Sources of cellulose 2
1.3 Hydrolysis 3
1.4 Statement of the problem 4
1.5 Relevance of the study 5
1.6 Objective and scope of the study 6
CHAPTER TWO
2.1 Literature review 7
2.2 History of cellulose 11
2.3 Occurences 12
2.4 Energy store of plants 13
2.5 Biosynthesis of cellulose 14
2.6 Structures of cellulose 15
2.7 Classifications of cellulose 16
2.7.1 Cellulose Nitrate 17
2.7.2 Cellulose Acetate 18
2.7.3 Cellulose Acetate Butyrate 19
2.7.4 Ethyl cellulose 20
2.7.5 Methyl cellulose 20
2.7.6 Carboxy Methyl cellulose 21
2.8 Hemicellulose 23
2.9 Breakdown (cellulolysis) 23
2.10 Functions of cellulose 24
2.11 Uses of sugar 25
2.12 Functions of sugar 27
2.13 Properties of cellulose 28
2.14 Methods of producing glucose from cellulose 29
CHAPTER THREE
3.0 Methodology 30
3.1 Materials and Equipment 30
3.1.1 Apparatus 30
3.2 Reagents 31
3.2.1 Hydrolysis (Acid hydrolysis) 32
3.2.2 Calorimetric Analysis of Glucose (using Benedict‟s solution) 33
CHAPTER FOUR
4.1 Results 35
4.2 Tables 36
4.3 Discussions 39
CHAPTER FIVE
5.1 Conclusion 41
5.2 Recommendation 42
References 43
Appendix I 45
Appendix II 47
List of Tables 49
List of figure 49
This “Kinetic Study On Hydrolysis Of Cellulose (Saw-Dust)” file contains 5 Chapters, 59 Pages and 2,399 Words.
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