Isolation And Characterization Of Micro-Organisms From Stored Pap (Ogi)
The microbial changes which took place during the steeping and storage of pan (ogi) was studied. The steeped water had an initial PH of 6.8 which latter reduced to 4.9 at the end of steeping. The bacterial number for the steeped water ranged from 4.7×104 to 3.2 x 107 cfu/ml while the fungal number ranged from 1.5 x103 to 5.7 x 106 cfu/ml. In the stored pap, pap I had higher count which range from 7.2×104 to 6.0×107 cfu/g while 6.0×107 to 1.6×1011. The fungal count for pap I and II ranged from 8.2 x 105 to 2.5×1012 and 8.2×106 to 3.6×1010 respectively. The bacteria isolated from stee pingwere Lactabacillus species Bacillus specie, Corynebacterium, streptococcus specie and clostridium species. The fungi were Aspergillus species fusrarium specie, pencillium specie sachanomyces specie and candida specie. The bacteria spp isolated from stored pap were lactobacillus species, streptococcus specie Eubacterium specie, Pseudomonas specie, Baccillus specie, streptococcus specie, Lactobacillus specie, Leucomostoc specie for pap I and II respectively. The fungi isolated were Aspergillus specie, Pensillum specie, fusarium specie, sacharomyces specie, candida specie, Debaryomyces specie for pap I and II respectively. The pap stored without changing water developed an off flavour after 48 hours and is not fit for consumption. On the other hand, the pap stored while changing water is fit and better for consumption.
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Title page
Certification
Dedication
Acknowledgement
Abstract
List of tables
List of figures
Table of contents
CHAPTER ONE
INTRODUCTION
1.1 Background of the study
1.2 Statement of problem
1.3 Aim and objectives of the study
1.4 Hypothesis
1.5 Justification of the study
1.6 Significance of study
1.7 Limitation of the study
CHAPTER TWO
LITERATURE
2.1 Origin of maize pad
2.2 Structure of maize grain
2.3 Chemical composition of maize grain
2.4 Uses of maize pap
2.5 Chemical changes in stored pap (ogi)
2.6 Nutrition changes in stored pap
2.7 Microorganisms associated with stored pap
CHAPTER THREE
MATERIALS AND METHOD
3.1 Collection of sample
3.2 Materials and equipments used
3.3 Media and reagents used
3.4 Preparation of pap (ogi)
3.5 Quantitative analysis of microorganisms
3.5.1 Serial dilution
3.5.2 Culturing technique
3.6 Isolation of bactria
3.7 Characterization of isolate
3.8 Biochemical test for identification of microorganisms
3.9 Identification of fungi
CHAPTER FOUR
RESULT AND DISCUSSION
CHAPTER FIVE
5.1 Conclusion
5.2 Recommendation
References
Appendix
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